| Rank | Czech as First Ancestry Population Percentage ▼ | School District / Population |
| 1. | 5.2% | Lincoln Plantation / 66 |
| 2. | 2.5% | Long Island / 261 |
| 3. | 1.5% | Bristol / 2,766 |
| 4. | 1.4% | Southport / 541 |
| 5. | 1.3% | Perry / 943 |
| 6. | 1.0% | Pembroke / 980 |
| 7. | 1.0% | Regional School Unit 24 / 19,161 |
| 8. | 0.8% | Dennysville / 399 |
| 9. | 0.8% | Otis / 400 |
| 10. | 0.8% | Penobscot / 1,377 |
| 10. | 0.8% | Jonesboro / 653 |
| 12. | 0.7% | Mount Desert / 1,708 |
| 13. | 0.7% | School Administrative District 60 / 17,650 |
| 13. | 0.7% | Regional School Unit 25 / 8,461 |
| 15. | 0.7% | Islesboro / 649 |
| 16. | 0.7% | Piscataquis Unorganized Territory / 936 |
| 17. | 0.6% | New Sweden / 513 |
| 18. | 0.6% | Woodland / 1,085 |
| 19. | 0.6% | School Administrative District 44 / 6,036 |
| 20. | 0.6% | Machiasport / 1,043 |
| 21. | 0.6% | School Administrative District 42 / 2,350 |
| 22. | 0.5% | School Administrative District 40 / 14,808 |
| 22. | 0.5% | Orrington / 3,710 |
| 24. | 0.5% | School Administrative District 63 / 6,002 |
| 25. | 0.5% | Brunswick / 20,557 |
| 25. | 0.5% | Auburn / 23,279 |
| 27. | 0.5% | Blue Hill / 2,649 |
| 28. | 0.4% | School Administrative District 75 / 19,689 |
| 29. | 0.4% | School Administrative District 41 / 4,842 |
| 30. | 0.4% | School Administrative District 49 / 14,919 |
| 31. | 0.4% | Tremont / 1,412 |
| 32. | 0.4% | Fayette / 1,281 |
| 33. | 0.3% | Regional School Unit 23 / 29,367 |
| 34. | 0.3% | South Portland / 24,804 |
| 34. | 0.3% | Regional School Unit 21 / 18,423 |
| 36. | 0.3% | School Administrative District 06 / 25,893 |
| 37. | 0.3% | Castine / 1,483 |
| 37. | 0.3% | Trenton / 1,453 |
| 37. | 0.3% | School Administrative District 03 / 10,528 |
| 40. | 0.3% | Kittery / 9,606 |
| 41. | 0.3% | Lewiston / 36,684 |
| 41. | 0.3% | School Administrative District 61 / 14,134 |
| 41. | 0.3% | School Administrative District 68 / 7,014 |
| 44. | 0.3% | Yarmouth / 8,390 |
| 44. | 0.3% | Wells-Ogunquit Community School District / 10,763 |
| 44. | 0.3% | Jefferson / 2,529 |
| 47. | 0.3% | Dedham / 2,139 |
| 48. | 0.2% | Regional School Unit 19 / 14,947 |
| 48. | 0.2% | Regional School Unit 13 / 17,358 |
| 50. | 0.2% | Cape Elizabeth / 9,056 |
| 51. | 0.2% | School Administrative District 53 / 6,142 |
| 51. | 0.2% | Vassalboro / 4,311 |
| 53. | 0.2% | Portland / 66,138 |
| 53. | 0.2% | School Administrative District 51 / 10,679 |
| 53. | 0.2% | Mount Desert Community School District / 10,039 |
| 53. | 0.2% | Regional School Unit 18 / 18,880 |
| 57. | 0.2% | Bangor / 32,899 |
| 57. | 0.2% | Regional School Unit 01 / 16,704 |
| 57. | 0.2% | York / 12,720 |
| 60. | 0.2% | School Administrative District 32 / 2,139 |
| 61. | 0.2% | School Administrative District 31 / 3,830 |
| 61. | 0.2% | School Administrative District 01 / 12,845 |
| 61. | 0.2% | Regional School Unit 14 / 21,108 |
| 61. | 0.2% | Lisbon / 9,092 |
| 65. | 0.1% | Regional School Unit 16 / 10,969 |
| 65. | 0.1% | Sanford / 21,035 |
| 65. | 0.1% | School Administrative District 27 / 6,683 |
| 65. | 0.1% | Brewer / 9,433 |
| 65. | 0.1% | School Administrative District 17 / 23,682 |
| 70. | 0.1% | Falmouth / 11,086 |
| 70. | 0.1% | School Administrative District 57 / 21,609 |
| 72. | 0.1% | Westbrook / 17,314 |
| 72. | 0.1% | Name N/A / 14,506 |
| 74. | 0.1% | School Administrative District 55 / 8,348 |
| 74. | 0.1% | School Administrative District 54 / 17,286 |
| 74. | 0.1% | School Administrative District 04 / 4,994 |
| 74. | 0.1% | Regional School Unit 04 / 10,221 |
| 78. | 0.1% | Regional School Unit 05 / 13,211 |
| 78. | 0.1% | Regional School Unit 38 / 7,458 |
| 80. | 0.1% | Biddeford / 21,490 |
| 80. | 0.1% | School Administrative District 09 / 18,036 |
| 80. | 0.1% | School Administrative District 35 / 13,431 |
| 80. | 0.1% | Regional School Unit 12 / 14,914 |
| 84. | 0.1% | School Administrative District 72 / 8,060 |
| 84. | 0.1% | Regional School Unit 26 / 16,708 |
| 84. | 0.1% | Gorham / 16,065 |
| 87. | 0.1% | Regional School Unit 02 / 14,748 |
| 87. | 0.1% | Waterville / 15,777 |
| 87. | 0.1% | School Administrative District 59 / 6,441 |
| 90. | 0.1% | School Administrative District 64 / 7,993 |
| 90. | 0.1% | School Administrative District 37 / 5,522 |
| 90. | 0.1% | School Administrative District 29 / 8,208 |
| 93. | 0.1% | Regional School Unit 20 / 18,312 |
| 94. | 0.0% | Regional School Unit 39 / 10,958 |
Please note that we only rank locations with 'Czech as First Ancestry Population Percentage' data. The rank above might not be a complete list. Locations without 'Czech as First Ancestry Population Percentage' data are not listed.
*ACS stands for U.S. Census American Community Survey. According to the U.S. Census, if the date is a range, you can interpret the data as an average of the period of time.
