| Rank | Czech as First Ancestry Population Percentage ▼ | School District / Population |
| 1. | 4.3% | Willimantic / 71 |
| 2. | 3.2% | Lincoln Plantation / 38 |
| 3. | 2.2% | Brewer / 9,452 |
| 4. | 2.1% | Pembroke / 931 |
| 5. | 1.5% | Bristol / 2,748 |
| 6. | 1.5% | Perry / 961 |
| 7. | 1.2% | Jonesboro / 511 |
| 8. | 1.1% | School Administrative District 63 / 6,369 |
| 9. | 1.1% | Hermon / 5,432 |
| 10. | 1.0% | Regional School Unit 24 / 18,720 |
| 11. | 1.0% | Robbinston / 579 |
| 12. | 0.8% | Aroostook Unorganized Territory / 1,408 |
| 13. | 0.7% | Name N/A / 1,779 |
| 14. | 0.7% | East Machias / 1,235 |
| 14. | 0.7% | School Administrative District 07 / 456 |
| 14. | 0.7% | School Administrative District 68 / 6,850 |
| 17. | 0.7% | Otis / 508 |
| 18. | 0.7% | School Administrative District 49 / 14,826 |
| 19. | 0.7% | East Range Community School District / 202 |
| 19. | 0.7% | Trenton / 1,419 |
| 21. | 0.6% | Islesboro / 626 |
| 22. | 0.6% | Baileyville / 1,392 |
| 23. | 0.6% | Southport / 539 |
| 24. | 0.5% | Piscataquis Unorganized Territory / 971 |
| 25. | 0.5% | Alexander / 419 |
| 25. | 0.5% | Vassalboro / 4,354 |
| 27. | 0.5% | Lisbon / 9,023 |
| 28. | 0.4% | Scarborough / 18,941 |
| 28. | 0.4% | South Portland / 24,997 |
| 28. | 0.4% | School Administrative District 03 / 10,466 |
| 28. | 0.4% | Penobscot / 1,086 |
| 32. | 0.4% | Machiasport / 1,161 |
| 33. | 0.4% | School Administrative District 06 / 25,994 |
| 33. | 0.4% | School Administrative District 27 / 6,495 |
| 35. | 0.4% | Regional School Unit 05 / 13,256 |
| 36. | 0.4% | Tremont / 1,775 |
| 36. | 0.4% | School Administrative District 60 / 17,903 |
| 38. | 0.3% | School Administrative District 75 / 19,564 |
| 38. | 0.3% | School Administrative District 51 / 10,796 |
| 38. | 0.3% | School Administrative District 35 / 13,454 |
| 41. | 0.3% | Regional School Unit 25 / 8,394 |
| 41. | 0.3% | School Administrative District 44 / 5,903 |
| 41. | 0.3% | Brooklin / 981 |
| 44. | 0.3% | Regional School Unit 01 / 16,270 |
| 45. | 0.3% | Dennysville / 408 |
| 45. | 0.3% | Regional School Unit 16 / 11,007 |
| 47. | 0.3% | School Administrative District 28 / 8,227 |
| 47. | 0.3% | Fayette / 1,203 |
| 49. | 0.3% | Castine / 1,673 |
| 49. | 0.3% | Acton / 2,457 |
| 49. | 0.3% | School Administrative District 01 / 13,023 |
| 52. | 0.3% | School Administrative District 40 / 14,537 |
| 53. | 0.3% | School Administrative District 41 / 4,454 |
| 54. | 0.2% | Portland / 66,235 |
| 54. | 0.2% | Brunswick / 20,376 |
| 56. | 0.2% | School Administrative District 55 / 8,061 |
| 57. | 0.2% | Lewiston / 36,588 |
| 57. | 0.2% | Gorham / 16,395 |
| 57. | 0.2% | School Administrative District 53 / 6,216 |
| 57. | 0.2% | Hope / 1,635 |
| 61. | 0.2% | Regional School Unit 20 / 18,290 |
| 62. | 0.2% | Regional School Unit 21 / 18,423 |
| 62. | 0.2% | Regional School Unit 23 / 29,430 |
| 62. | 0.2% | Penobscot Unorganized Territory / 1,192 |
| 62. | 0.2% | Name N/A / 13,696 |
| 66. | 0.2% | School Administrative District 24 / 2,980 |
| 66. | 0.2% | Regional School Unit 26 / 17,096 |
| 66. | 0.2% | School Administrative District 37 / 5,873 |
| 69. | 0.2% | Auburn / 23,104 |
| 70. | 0.2% | School Administrative District 17 / 24,188 |
| 70. | 0.2% | Regional School Unit 19 / 14,814 |
| 70. | 0.2% | Regional School Unit 18 / 19,006 |
| 73. | 0.1% | Regional School Unit 13 / 17,136 |
| 73. | 0.1% | School Administrative District 61 / 14,444 |
| 73. | 0.1% | Regional School Unit 04 / 10,431 |
| 76. | 0.1% | Cape Elizabeth / 9,055 |
| 76. | 0.1% | School Administrative District 72 / 7,759 |
| 76. | 0.1% | School Administrative District 74 / 5,330 |
| 76. | 0.1% | School Administrative District 64 / 7,793 |
| 80. | 0.1% | York / 12,625 |
| 80. | 0.1% | Winslow / 7,779 |
| 80. | 0.1% | Dedham / 1,850 |
| 80. | 0.1% | Kittery / 9,538 |
| 84. | 0.1% | Regional School Unit 38 / 7,758 |
| 84. | 0.1% | Name N/A / 31,164 |
| 86. | 0.1% | School Administrative District 23 / 5,610 |
| 86. | 0.1% | Regional School Unit 02 / 14,802 |
| 86. | 0.1% | School Administrative District 32 / 2,159 |
| 89. | 0.1% | Bangor / 32,988 |
| 89. | 0.1% | School Administrative District 54 / 17,259 |
| 89. | 0.1% | Regional School Unit 39 / 10,596 |
| 89. | 0.1% | School Administrative District 04 / 5,172 |
| 93. | 0.1% | School Administrative District 29 / 8,199 |
| 93. | 0.1% | School Administrative District 22 / 12,465 |
| 93. | 0.1% | Waterville / 15,780 |
| 93. | 0.1% | Westbrook / 17,501 |
| 97. | 0.1% | Regional School Unit 12 / 14,737 |
| 97. | 0.1% | Mount Desert Community School District / 10,939 |
| 99. | 0.1% | Regional School Unit 14 / 21,475 |
| 99. | 0.1% | School Administrative District 09 / 17,751 |
| 101. | 0.0% | School Administrative District 46 / 6,550 |
| 101. | 0.0% | Sanford / 20,885 |
| 103. | 0.0% | School Administrative District 31 / 4,033 |
| 103. | 0.0% | Biddeford / 21,351 |
| 105. | 0.0% | School Administrative District 57 / 21,912 |
Please note that we only rank locations with 'Czech as First Ancestry Population Percentage' data. The rank above might not be a complete list. Locations without 'Czech as First Ancestry Population Percentage' data are not listed.
*ACS stands for U.S. Census American Community Survey. According to the U.S. Census, if the date is a range, you can interpret the data as an average of the period of time.
