| Rank | Czech as First Ancestry Population Percentage ▼ | School District / Population |
| 1. | 0.9% | Suwannee County School District / 43,404 |
| 2. | 0.8% | Sumter County School District / 103,708 |
| 3. | 0.7% | Monroe County School District / 75,208 |
| 3. | 0.7% | Glades County School District / 13,190 |
| 5. | 0.6% | Pinellas County School District / 925,030 |
| 6. | 0.6% | Brevard County School District / 548,891 |
| 7. | 0.6% | Sarasota County School District / 386,944 |
| 7. | 0.6% | Manatee County School District / 335,840 |
| 9. | 0.6% | Flagler County School District / 98,843 |
| 10. | 0.6% | St. Johns County School District / 203,402 |
| 11. | 0.5% | Collier County School District / 334,474 |
| 11. | 0.5% | Hernando County School District / 173,792 |
| 13. | 0.5% | Lee County School District / 647,554 |
| 14. | 0.5% | Charlotte County School District / 163,151 |
| 14. | 0.5% | Wakulla County School District / 31,015 |
| 16. | 0.5% | Martin County School District / 149,658 |
| 17. | 0.4% | Nassau County School District / 74,918 |
| 18. | 0.4% | Lake County School District / 305,010 |
| 19. | 0.4% | Palm Beach County School District / 1,359,074 |
| 19. | 0.4% | Marion County School District / 334,771 |
| 21. | 0.4% | Okaloosa County School District / 188,984 |
| 21. | 0.4% | Bay County School District / 172,988 |
| 21. | 0.4% | Hardee County School District / 27,549 |
| 24. | 0.4% | Seminole County School District / 432,135 |
| 24. | 0.4% | Hamilton County School District / 14,466 |
| 24. | 0.4% | Clay County School District / 194,868 |
| 24. | 0.4% | Pasco County School District / 472,745 |
| 24. | 0.4% | Citrus County School District / 139,771 |
| 29. | 0.4% | Indian River County School District / 140,918 |
| 29. | 0.4% | Highlands County School District / 98,261 |
| 31. | 0.4% | Washington County School District / 24,660 |
| 31. | 0.4% | Leon County School District / 280,882 |
| 31. | 0.4% | Alachua County School District / 251,759 |
| 31. | 0.4% | Jefferson County School District / 14,335 |
| 35. | 0.3% | Volusia County School District / 498,981 |
| 35. | 0.3% | Santa Rosa County School District / 158,240 |
| 35. | 0.3% | Hillsborough County School District / 1,279,668 |
| 38. | 0.3% | Escambia County School District / 304,099 |
| 39. | 0.3% | Orange County School District / 1,200,241 |
| 40. | 0.3% | Putnam County School District / 73,191 |
| 41. | 0.3% | St. Lucie County School District / 283,988 |
| 42. | 0.3% | Levy County School District / 40,057 |
| 43. | 0.3% | DeSoto County School District / 34,785 |
| 43. | 0.3% | Broward County School District / 1,815,269 |
| 43. | 0.3% | Franklin County School District / 11,636 |
| 43. | 0.3% | Walton County School District / 57,821 |
| 47. | 0.2% | Duval County School District / 880,750 |
| 48. | 0.2% | Madison County School District / 18,901 |
| 49. | 0.2% | Polk County School District / 617,323 |
| 50. | 0.2% | Union County School District / 15,258 |
| 51. | 0.2% | Lafayette County School District / 8,821 |
| 52. | 0.2% | Gilchrist County School District / 16,948 |
| 52. | 0.2% | Jackson County School District / 49,105 |
| 52. | 0.2% | Osceola County School District / 289,449 |
| 55. | 0.2% | Columbia County School District / 67,662 |
| 56. | 0.1% | Dade County School District / 2,600,861 |
| 57. | 0.1% | Holmes County School District / 19,741 |
| 58. | 0.1% | Okeechobee County School District / 39,398 |
| 59. | 0.1% | Calhoun County School District / 14,657 |
| 59. | 0.1% | Gadsden County School District / 46,865 |
| 61. | 0.1% | Liberty County School District / 8,302 |
| 62. | 0.0% | Baker County School District / 27,057 |
| 62. | 0.0% | Dixie County School District / 16,137 |
Please note that we only rank locations with 'Czech as First Ancestry Population Percentage' data. The rank above might not be a complete list. Locations without 'Czech as First Ancestry Population Percentage' data are not listed.
